BD-PB2456-PAX6 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

PAX6 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB2456

产品名称

PAX6 Rabbit Polyclonal Antibody (Center)

别名

Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6, AN2

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_000271.1;NP_001121084.1;NP_001245393.1;NP_001245394.1;NP_001595.2

Human Swissprot No.

P26367

特异性

This PAX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-210 amino acids from the Central region of human PAX6.

稀释度

WB~~1:1000;IHC-P~~1:100~500;IF~~1:10~50;FC~~1:10~50

预测分子量

47kDa

同种型

Rabbit Ig

背景介绍

PAX6 is paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription.

组织表达

[Isoform 1]: Expressed in lymphoblasts.

细胞定位

Nucleus {ECO:0000250|UniProtKB:P63015}. [Isoform 5a]: Nucleus {ECO:0000250|UniProtKB:P63016}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

PAX6 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PAX6 antibody detected the PAX6 protein (arrow).

Formalin-fixed and paraffin-embedded human brain tissue with PAX6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of PAX6 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Flow cytometric analysis of hela cells using PAX6 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.