BD-PB2455-SRP72 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

SRP72 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB2455

产品名称

SRP72 Rabbit Polyclonal Antibody (Center)

别名

Signal recognition particle subunit SRP72, SRP72, Signal recognition particle 72 kDa protein, SRP72

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001254651.1;NP_008878.3

Human Swissprot No.

O76094

特异性

This SRP72 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the Central region of human SRP72.

稀释度

WB~~1:1000;FC~~1:10~50;IHC-P~~1:100~500;IF~~1:10~50

预测分子量

75kDa

同种型

Rabbit Ig

背景介绍

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. It binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.

细胞定位

Cytoplasm. Endoplasmic reticulum

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-SRP72 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Flow cytometric analysis of hela cells using SRP72 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human pancreas with SRP72 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence analysis of SRP72 Antibody (Center) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.