BD-PB2454-HLA-DRB1 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

HLA-DRB1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2454

产品名称

HLA-DRB1 Rabbit Polyclonal Antibody (N-term)

别名

HLA class II histocompatibility antigen, DRB1-1 beta chain, MHC class II antigen DRB1*1, DR-1, DR1, HLA-DRB1

类别

常规抗体

推荐应用

反应种属

Human

Human Swissprot No.

P04229

特异性

This HLA-D_1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human HLA-D_1.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

同种型

Rabbit Ig

背景介绍

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Western blot analysis of lysates from NCI-H1299, Raji cell line (from left to right), using HLA-DRB1 Antibody (N-term). BD-PB2454 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot analysis of HLA-DRB1 Antibody (N-term) in WiDr cell line lysates (35ug/lane). HLA-DRB1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with HLA-DRB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of widr cells using HLA-DRB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.