BD-PB2453-GALT Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

GALT Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2453

产品名称

GALT Rabbit Polyclonal Antibody (C-term)

别名

Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase, GALT

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_000146.2;NP_001245261.1

Human Swissprot No.

P07902

特异性

This GALT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-373 amino acids from the C-terminal region of human GALT.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

43kDa

同种型

Rabbit Ig

背景介绍

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-GALT Antibody (C-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of GALT Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). GALT (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with GALT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of MDA-MB468 cells using GALT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.