BD-PB2451-ESPN Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

ESPN Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2451

产品名称

ESPN Rabbit Polyclonal Antibody (N-term)

别名

Espin, Autosomal recessive deafness type 36 protein, Ectoplasmic specialization protein, ESPN, DFNB36

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_113663.2

Human Swissprot No.

B1AK53

特异性

This ESPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human ESPN.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

92kDa

同种型

Rabbit Ig

背景介绍

ESPN is a multifunctional actin-bundling protein. It plays a major role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells.

细胞定位

Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q9ET47}. Cell projection, stereocilium. Cell projection, microvillus

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-ESPN Antibody (N-term) at 1:1000 dilution + SK-BR-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 92 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of ESPN Antibody (N-term) in 293 cell line lysates (35ug/lane). ESPN (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with ESPN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ESPN Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.