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ENTH Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号
BD-PB2447
产品名称
ENTH Rabbit Polyclonal Antibody (Center)
别名
Clathrin interactor 1, Clathrin-interacting protein localized in the trans-Golgi region, Clint, Enthoprotin, Epsin-4, Epsin-related protein, EpsinR, CLINT1, ENTH, EPN4, EPNR, KIAA0171
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_001182484.1;NP_001182485.1;NP_055481.1
Human Swissprot No.
Q14677
特异性
This ENTH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-249 amino acids from the Central region of human ENTH.
稀释度
WB~~1:1000;IHC-P~~1:100~500
预测分子量
68kDa
同种型
Rabbit Ig
背景介绍
ENTH binds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). It May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. It stimulates clathrin assembly.
组织表达
Ubiquitously expressed at low to intermediate levels.
细胞定位
Cytoplasm. Cytoplasm, perinuclear region. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, clathrin- coated vesicle. Note=Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Western blot analysis of ENTH Antibody (Center) in HL-60 cell line lysates (35ug/lane). ENTH (arrow) was detected using the purified Pab.

(LEFT)Formalin-fixed and paraffin-embedded human brain with ENTH Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT)Flow cytometric analysis of NCI-H292 cells using ENTH Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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