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ALKBH8 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB2439
产品名称
ALKBH8 Rabbit Polyclonal Antibody (C-term)
别名
Alkylated DNA repair protein alkB homolog 8, 11411-, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8, tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8, ALKBH8, ABH8
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_620130.2
Human Swissprot No.
Q96BT7
特异性
This ALKBH8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from the C-terminal region of human ALKBH8.
稀释度
WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50
预测分子量
75kDa
同种型
Rabbit Ig
背景介绍
ALKBH8 may inhibit apoptosis and promote cell survival and angiogenesis.
组织表达
Widely expressed, with highest expression in spleen, followed by pancreas and lung.
细胞定位
Cytoplasm. Nucleus Note=Predominantly cytoplasmic.
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Anti-ALKBH8 Antibody (C-term) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H292 cells using ALKBH8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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