BD-PB2430-AMT Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

AMT Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2430

产品名称

AMT Rabbit Polyclonal Antibody (N-term)

别名

Aminomethyltransferase, mitochondrial, Glycine cleavage system T protein, GCVT, AMT, GCST

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_000472.2;NP_001158182.1;NP_001158183.1;NP_001158184.1

Human Swissprot No.

P48728

特异性

This AMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-45 amino acids from the N-terminal region of human AMT.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

44kDa

同种型

Rabbit Ig

背景介绍

The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.

细胞定位

Mitochondrion.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Western blot analysis of AMT Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AMT (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AMT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

AMT Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.