BD-PB2414-GLO1 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

GLO1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2414

产品名称

GLO1 Rabbit Polyclonal Antibody (N-term)

别名

Lactoylglutathione lyase, Aldoketomutase, Glyoxalase I, Glx I, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase, GLO1

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_006699.2

Human Swissprot No.

Q04760

特异性

This GLO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human GLO1.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50;IF~~1:10~50

预测分子量

21kDa

同种型

Rabbit Ig

背景介绍

The enzyme GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Western blot analysis of GLO1 antibody (N-term) in HL60 cell line lysates (35ug/lane). GLO1 (arrow) was detected using the purified Pab.

All lanes : Anti-GLO1 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human brain tissue reacted with GLO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of widr cells using GLO1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.

Confocal immunofluorescent analysis of GLO1 Antibody (N-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).