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EMILIN1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号
BD-PB2410
产品名称
EMILIN1 Rabbit Polyclonal Antibody (N-term)
别名
EMILIN-1, Elastin microfibril interface-located protein 1, Elastin microfibril interfacer 1, EMILIN1, EMI
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_008977.1
Human Swissprot No.
Q9Y6C2
特异性
This EMILIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-112 amino acids from the N-terminal region of human EMILIN1.
稀释度
WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50
预测分子量
107kDa
同种型
Rabbit Ig
背景介绍
EMILIN1 may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity.
组织表达
Distributed in tissues where resilience and elastic recoil are prominent. Highest levels in the adult small intestine, aorta, lung, uterus, and appendix and in the fetal spleen, kidney, lung, and heart; intermediate expression was detected in adult liver, ovary, colon, stomach, lymph node and spleen; adult heart, bladder, prostate, adrenal gland, mammary gland, placenta and kidney showed low expression whereas a series of other adult tissues, including skeletal muscle and different regions of adult brain show no expression
细胞定位
Secreted, extracellular space, extracellular matrix. Note=Found mainly at the interface between amorphous elastin and microfibrils
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Western blot analysis of EMILIN1 Antibody (N-term) in K562 and 293 cell line lysates (35ug/lane). EMILIN1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle with EMILIN1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of 293 cells using EMILIN1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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