BD-PB2406-FIBG Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

FIBG Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2406

产品名称

FIBG Rabbit Polyclonal Antibody (C-term)

别名

Fibrinogen gamma chain, FGG

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_000500.2;NP_068656.2

Human Swissprot No.

P02679

特异性

This FIBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-449 amino acids from the C-terminal region of human FIBG.

稀释度

WB~~1:1000;IHC-P~~1:100~500;IF~~1:10~50

预测分子量

52kDa

同种型

Rabbit Ig

背景介绍

FIBG is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in its gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.

组织表达

Detected in blood plasma (at protein level).

细胞定位

Secreted

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Anti-FIBG Antibody (C-term) at 1:4000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of FIBG Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). FIBG (arrow) was detected using the purified Pab.

FIBG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FIBG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of FIBG Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).