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ACTR2 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB2400
产品名称
ACTR2 Rabbit Polyclonal Antibody (C-term)
别名
Actin-related protein 2, Actin-like protein 2, ACTR2, ARP2
类别
常规抗体
推荐应用
WB
反应种属
Human, Rat, Mouse
Human Gene ID
NP_001005386.1;NP_005713.1
Human Swissprot No.
P61160
特异性
This ACTR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-352 amino acids from the C-terminal region of human ACTR2.
稀释度
WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50
预测分子量
45kDa
同种型
Rabbit Ig
背景介绍
ACTR2 is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.
细胞定位
Cytoplasm, cytoskeleton. Cell projection. Nucleus
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

All lanes : Anti-ACTR2 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ACTR2 Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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