BD-PB2398-ARPC1A Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

ARPC1A Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2398

产品名称

ARPC1A Rabbit Polyclonal Antibody (C-term)

别名

Actin-related protein 2/3 complex subunit 1A, SOP2-like protein, ARPC1A, SOP2L

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_006400.2

Human Swissprot No.

Q92747

特异性

This ARPC1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human ARPC1A.

稀释度

WB~~1:1000;IHC-P~~1:100~500;IF~~1:10~50;FC~~1:10~50

预测分子量

42kDa

同种型

Rabbit Ig

背景介绍

ARPC1A is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.

细胞定位

Cytoplasm, cytoskeleton. Nucleus {ECO:0000250|UniProtKB:Q8AVT9}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Anti-ARPC1A Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ARPC1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence analysis of ARPC1A Antibody (C-term) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Flow cytometric analysis of WiDr cells using ARPC1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.