BD-PB2397-CAV3 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

CAV3 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2397

产品名称

CAV3 Rabbit Polyclonal Antibody (N-term)

别名

Caveolin-3, M-caveolin, CAV3

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_001225.1;NP_203123.1

Human Swissprot No.

P56539

特异性

This CAV3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-31 amino acids from the N-terminal region of human CAV3.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

17kDa

同种型

Rabbit Ig

背景介绍

CAV3 is a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in its gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD).

组织表达

Expressed predominantly in muscle.

细胞定位

Golgi apparatus membrane; Peripheral membrane protein. Cell membrane {ECO:0000250|UniProtKB:P51638}; Peripheral membrane protein. Membrane, caveola {ECO:0000250|UniProtKB:P51637}; Peripheral membrane protein. Cell membrane, sarcolemma {ECO:0000250|UniProtKB:P51637}. Note=Potential hairpin-like structure in the membrane. Membrane protein of caveolae (By similarity)

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Anti-CAV3 Antibody (N-term)at 1:500 dilution + human normal uterus lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of CAV3 antibody (N-term) in 293 cell line lysates (35ug/lane). CAV3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with CAV3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of 293 cells using CAV3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.