BD-PB2393-IL1RN Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

IL1RN Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2393

产品名称

IL1RN Rabbit Polyclonal Antibody (C-term)

别名

Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, Anakinra, IL1RN, IL1F3, IL1RA

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_000568.1;NP_776213.1;NP_776214.1;NP_776215.1

Human Swissprot No.

P18510

特异性

This IL1RN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-177 amino acids from the C-terminal region of human IL1RN.

稀释度

WB~~1:1000;FC~~1:10~50;IF~~1:10~50

预测分子量

20kDa

同种型

Rabbit Ig

背景介绍

The protein is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of its gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer.

组织表达

The intracellular form of IL1RN is predominantly expressed in epithelial cells

细胞定位

[Isoform 1]: Secreted. [Isoform 3]: Cytoplasm.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Anti-IL1RN Antibody (C-term) at 1:4000 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

IL1RN Antibody (C-term) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the IL1RN antibody detected the IL1RN protein (arrow).

IL1RN Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of IL1RN Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).