产品名称
Myosin 1C (MYO1C) Rabbit Polyclonal Antibody (C-term)
别名
Unconventional myosin-Ic, Myosin I beta, MMI-beta, MMIb, MYO1C
Human Gene ID
NP_001074248.1;NP_001074419.1;NP_203693.3
Human Swissprot No.
O00159
特异性
This Myosin 1C (MYO1C) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 911-940 amino acids from the C-terminal region of human Myosin 1C (MYO1C).
稀释度
WB~~1:1000;IHC-P~~1:100~500
背景介绍
MYO1C is a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane.
细胞定位
[Isoform 1]: Cytoplasm. Nucleus Note=Colocalizes with RNA polymerase II. Absent from nucleoli and does not colocalize with RNA polymerase I. Translocates to nuclear speckles upon exposure to inhibitors of RNA polymerase II transcription [Isoform 3]: Nucleus, nucleoplasm. Nucleus, nucleolus. Nucleus, nuclear pore complex. Note=Colocalizes with RNA polymerase II in the nucleus. Colocalizes with RNA polymerase I in nucleoli (By similarity). In the nucleolus, is localized predominantly in dense fibrillar component (DFC) and in granular component (GC) Accumulates strongly in DFC and GC during activation of transcription Colocalizes with transcription sites. Colocalizes in the granular cortex at the periphery of the nucleolus with RPS6. Colocalizes in nucleoplasm with RPS6 and actin that are in contact with RNP particles Colocalizes with RPS6 at the nuclear pore level.
纯化
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
