BD-PB2377-SIRT6 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

SIRT6 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2377

产品名称

SIRT6 Rabbit Polyclonal Antibody (C-term)

别名

NAD-dependent protein deacetylase sirtuin-6, 351-, Regulatory protein SIR2 homolog 6, SIR2-like protein 6, SIRT6, SIR2L6

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_001180214.1;NP_057623.2

Human Swissprot No.

Q8N6T7

特异性

This SIRT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-319 amino acids from the C-terminal region of human SIRT6.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

39kDa

同种型

Rabbit Ig

背景介绍

SIRT6 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

细胞定位

Nucleus, nucleoplasm. Note=Predominantly nuclear. Associated with telomeric heterochromatin regions

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Anti-SIRT6 Antibody (C-term) at 1:500 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of SIRT6 Antibody (C-term) in mouse stomach tissue lysates (35ug/lane). SIRT6 (arrow) was detected using the purified Pab.