BD-PB2372-NOTCH3 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

NOTCH3 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2372

产品名称

NOTCH3 Rabbit Polyclonal Antibody (C-term)

别名

Neurogenic locus notch homolog protein 3, Notch 3, Notch 3 extracellular truncation, Notch 3 intracellular domain, NOTCH3

类别

常规抗体

推荐应用

反应种属

Mouse, Rat, Human

Human Gene ID

NP_000426.2

Human Swissprot No.

Q9UM47

特异性

This NOTCH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2291-2321 amino acids from the C-terminal region of human NOTCH3.

稀释度

WB~~1:1000;IF~~1:10~50;IHC-P~~1:100~500

预测分子量

244kDa

同种型

Rabbit Ig

背景介绍

NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

组织表达

Ubiquitously expressed in fetal and adult tissues.

细胞定位

Cell membrane; Single-pass type I membrane protein

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

NOTCH3-Q2306 western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the NOTCH3 antibody detected the NOTCH3 protein (arrow).

Confocal immunofluorescent analysis of NOTCH3 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.