BD-PB2368-GREMLIN Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

GREMLIN Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2368

产品名称

GREMLIN Rabbit Polyclonal Antibody (C-term)

别名

Gremlin-1, Cysteine knot superfamily 1, BMP antagonist 1, Down-regulated in Mos-transformed cells protein, Grem1, Cktsf1b1, Drm

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

O70326

Mouse Gene ID

NP_035954.1

特异性

This GREMLIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the C-terminal region of human GREMLIN.

稀释度

IHC-P~~1~200;WB~~1:2000

预测分子量

21kDa

同种型

Rabbit Ig

背景介绍

GREMLIN is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth.

组织表达

Highly expressed in spleen and to a lesser extent in lung, skeletal muscle and kidney. Expressed only in non-transformed cells or primary fibroblasts in culture but not in established transformed or tumor derived cell lines. Broadly expressed in limb bud mesenchyme but restricted to the distal limb bud mesenchyme and concentrated posteriorly. Expressed in ovary especially in granulosa cells of follicles of type 4.

细胞定位

Secreted.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunohistochemical analysis of paraffin-embedded mouse lung section using GREMLIN Antibody (C-term) . BD-PB2368 was diluted at 1:200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded mouse spleen section using GREMLIN Antibody (C-term) . BD-PB2368 was diluted at 1:200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded human spleen section using GREMLIN Antibody (C-term) . BD-PB2368 was diluted at 1:200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded mouse kidney section using GREMLIN Antibody (C-term) . BD-PB2368 was diluted at 1:200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Anti-GREMLIN Antibody (C-term) at 1:2000 dilution + Mouse skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.