产品名称
INP5E Rabbit Polyclonal Antibody
蛋白名称
72 kDa inositol polyphosphate 5-phosphatase (EC 3.1.3.36) (Phosphatidylinositol 4,5-bisphosphate 5-phosphatase) (Phosphatidylinositol polyphosphate 5-phosphatase type IV)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9NRR6
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9NRR6/entry
Mouse Swissprot No.
Q9JII1
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JII1
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q9WVR1
免疫原
Synthesized peptide derived from human protein . at AA range: 490-570
特异性
INP5E Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. Studies of the mouse counterpart suggest that this protein may hydrolyze phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,5-bisphosphate on the cytoplasmic Golgi membrane and thereby regulate Golgi-vesicular trafficking. Mutations in this gene cause Joubert syndrome; a clinically and genetically heterogenous group of disorders characterized by midbrain-hindbrain malformation and various associated ciliopathies that include retinal dystrophy, nephronophthisis, liver fibrosis and polydactyly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016],
组织表达
Detected in brain, heart, pancreas, testis and spleen.
细胞定位
Cytoplasm, cytoskeleton, cilium axoneme . Golgi apparatus, Golgi stack membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, ruffle . Cytoplasm . Nucleus . Peripheral membrane protein associated with Golgi stacks. .
信号通路
Inositol phosphate metabolism;Phosphatidylinositol signaling system;
功能
catalytic activity:1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.,function:Converts phosphatidylinositol-3,4,5-triphosphate (PtdIns 3,4,5-P3) to PtdIns-P2. Specific for lipid substrates, inactive towards water soluble inositol phosphates.,miscellaneous:Active in the presence of octyl-glucoside or Triton X-100, but completely inhibited by CTAB.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Several sequencing problems.,similarity:Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type IV family.,subcellular location:Peripheral membrane protein associated with Golgi stacks.,tissue specificity:Detected in brain, heart, pancreas, testis and spleen.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
