Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling ARHGAP39 with BD-PB2349 at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus and Weak Cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).