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ENAM Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB2338
产品名称
ENAM Rabbit Polyclonal Antibody (C-term)
别名
Enamelin, ENAM
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_114095.2
Human Swissprot No.
Q9NRM1
特异性
This ENAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1097-1126 amino acids from the C-terminal region of human ENAM.
稀释度
WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50
预测分子量
129kDa
同种型
Rabbit Ig
组织表达
Expressed in tooth particularly in odontoblast, ameloblast and cementoblast.
细胞定位
Secreted, extracellular space, extracellular matrix {ECO:0000250|UniProtKB:O97939}
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-ENAM Antibody (C-term) at 1:1000 dilution Lane 1: U-87 MG whole cell lysate Lane 2: K562 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size : 95kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-ENAM Antibody (C-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size : 95kDa Blocking/Dilution buffer: 5% NFDM/TBST.

ENAM Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ENAM antibody detected the ENAM protein (arrow).

ENAM antibody(C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENAM antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ENAM Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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