产品名称
8ODP Rabbit Polyclonal Antibody
蛋白名称
7,8-dihydro-8-oxoguanine triphosphatase (EC 3.6.1.55) (2-hydroxy-dATP diphosphatase) (EC 3.6.1.56) (8-oxo-dGTPase) (Nucleoside diphosphate-linked moiety X motif 1) (Nudix motif 1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P36639
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P36639/entry
Mouse Swissprot No.
P53368
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P53368
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P53369
免疫原
Synthesized peptide derived from human protein . at AA range: 1-80
特异性
8ODP Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer is
组织表达
Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes.
细胞定位
[Isoform p18]: Cytoplasm, cytosol . Mitochondrion matrix . Nucleus . Mostly present in cytosol (PubMed:7782328). A minor proportion is mitochondrial (PubMed:7782328). A very small amount of the protein is associated with nuclei (PubMed:7782328). .; [Isoform p26]: Mitochondrion matrix .
功能
catalytic activity:8-oxo-dGTP + H(2)O = 8-oxo-dGMP + diphosphate.,developmental stage:In peripheral blood lymphocytes, expressed at much higher levels in proliferating cells than in resting cells.,function:Antimutagenic. Responsible for preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions.,polymorphism:A polymorphism between Met-1 and Met-19 removes a stop codon before the initiation codon for isoform p22 and gives rise to the production of isoform p26. The allele frequency of isoform p26 is about 20%.,PTM:The N-terminus is blocked.,similarity:Belongs to the Nudix hydrolase family.,tissue specificity:Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
