BD-PB2322-WNT5A Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

WNT5A Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB2322

产品名称

WNT5A Rabbit Polyclonal Antibody (Center)

别名

Protein Wnt-5a, WNT5A

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_001243034.1;NP_003383.2

Human Swissprot No.

P41221

特异性

This WNT5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-213 amino acids from the Central region of human WNT5A.

稀释度

WB~~1:2000;IHC-P-Leica~~1:100;FC~~1:25

预测分子量

42kDa

同种型

Rabbit Ig

背景介绍

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq].

组织表达

Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level) (PubMed:15735754). Detected in neonate heart and lung (PubMed:8288227)

细胞定位

Secreted, extracellular space, extracellular matrix. Secreted

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes: Anti-WNT5A Antibody (Center) at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: Rat heart lysate Lane 3: mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated E42at 1/15000 dilution. Observed band size: 42KDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue using BD-PB2322 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes : Anti-WNT5A Antibody (Center) at 1:500 dilution Lane 1: Mouse heart lysate Lane 2: Rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Hela cells stained with BD-PB2322(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-WNT5A Antibody (Center) at 1:500 dilution Lane 1: Hela whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse heart lysate Lane 4: PANC-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-WNT5A Antibody (Center) at 1:2000 dilution Lane 1: human heart lysate Lane 2: PANC-1 whole cell lysate Lane 3: mouse heart lysate Lane 4: rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.