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HHAT Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号
BD-PB2306
产品名称
HHAT Rabbit Polyclonal Antibody (N-term)
别名
Protein-cysteine N-palmitoyltransferase HHAT, 231-, Hedgehog acyltransferase, Melanoma antigen recognized by T-cells 2, MART-2, Skinny hedgehog protein 1, HHAT, MART2, SKI1
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_001116306.1;NP_001164035.1;NP_001164051.1;NP_001164058.1;NP_001164059.1;NP_060664.2
Human Swissprot No.
Q5VTY9
特异性
This HHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human HHAT.
稀释度
WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50
预测分子量
57kDa
同种型
Rabbit Ig
背景介绍
'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725).
组织表达
Ubiquitously expressed in normal tissues and cancer cell lines.
细胞定位
Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Co-localizes with SHH in the ER and Golgi membrane.
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

HHAT Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HHAT antibody detected the HHAT protein (arrow).

HHAT Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HHAT Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

HHAT Antibody (N-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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