BD-PB2278-MFRN2 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

MFRN2 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2278

产品名称

MFRN2 Rabbit Polyclonal Antibody (N-term)

别名

Mitoferrin-2, Mitochondrial RNA-splicing protein 3/4 homolog, MRS3/4, hMRS3/4, Mitochondrial iron transporter 2, Solute carrier family 25 member 28, SLC25A28, MFRN2

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_112489.3

Human Swissprot No.

Q96A46

特异性

This MFRN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-64 amino acids from the N-terminal region of human MFRN2.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

39kDa

同种型

Rabbit Ig

背景介绍

MFRN2 is mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme.

组织表达

Ubiquitous. Expressed in placenta, lung, kidney, pancreas, liver, brain, skeletal muscle and heart

细胞定位

Mitochondrion inner membrane; Multi-pass membrane protein. Note=Isoform 1 and isoform 2 are both localized in the mitochondrion

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-MFRN2 Antibody (N-term) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of MFRN2 Antibody (N-term) in Jurkat cell line lysates (35ug/lane).MFRN2 (arrow) was detected using the purified Pab.

MFRN2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MFRN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

MFRN2 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.