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HIST1H1B Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号
BD-PB2275
产品名称
HIST1H1B Rabbit Polyclonal Antibody (N-term)
别名
Histone H15, Histone H1a, Histone H1b, Histone H1s-3, HIST1H1B, H1F5
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_005313.1
Human Swissprot No.
P16401
特异性
This HIST1H1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 amino acids from the N-terminal region of human HIST1H1B.
稀释度
WB~~1:1000;FC~~1:10~50
预测分子量
23kDa
同种型
Rabbit Ig
背景介绍
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family.
组织表达
Ubiquitous. Expressed in the majority of the cell lines tested and in testis.
细胞定位
Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-HIST1H1B Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

HIST1H1B Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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