BD-PB2270-ERO1LB Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

ERO1LB Rabbit Polyclonal Antibody (C-term)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB2270

产品名称

ERO1LB Rabbit Polyclonal Antibody (C-term)

别名

ERO1-like protein beta, ERO1-L-beta, 184-, Endoplasmic reticulum oxidoreductin-1-like protein B, Oxidoreductin-1-L-beta, ERO1LB

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_063944.3

Human Swissprot No.

Q86YB8

特异性

This ERO1LB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-293 amino acids from the C-terminal region of human ERO1LB.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

54kDa

同种型

Rabbit Ig

背景介绍

ERO1LB is essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1LB acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. ERO1LB does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent.

组织表达

Highly expressed in the digestive tract, including the duodenum and lower digestive tract. In the stomach, highly expressed in enzyme-producing chief cells (at protein level). In the pancreas, expressed in islets of Langerhans and, at lower levels, in enzyme-secreting cells (at protein level). Detected at low level in many other tissues.

细胞定位

Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side. Note=The association with ERP44 may be essential for its retention in the endoplasmic reticulum

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

ERO1LB Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the ERO1LB antibody detected the ERO1LB protein (arrow).

ERO1LB Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERO1LB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ERO1LB Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.