BD-PB2249-FBXL2 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

FBXL2 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2249

产品名称

FBXL2 Rabbit Polyclonal Antibody (C-term)

别名

F-box/LRR-repeat protein 2, F-box and leucine-rich repeat protein 2 {ECO:0000312|HGNC:HGNC:13598}, F-box protein FBL2/FBL3 {ECO:0000303|PubMed:10945468, ECO:0000312|EMBL:AAF045101}, FBXL2 (HGNC:13598)

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_001165184.1;NP_036289.3

Human Swissprot No.

Q9UKC9

特异性

This FBXL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-395 amino acids from the C-terminal region of human FBXL2.

稀释度

WB~~1:1000-1:2000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

47kDa

同种型

Rabbit Ig

背景介绍

FBXL2 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats.

组织表达

Expressed in brain, heart, kidney, liver, lung, pancreas and placenta.

细胞定位

Membrane; Lipid- anchor

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-FBXL2 Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: Human heart lysate Lane 2: Human liver lysate Lane 3: Hela whole cell lysate Lane 4: MDA-MB-453 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

FBXL2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

FBXL2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.