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FUT6 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号
BD-PB2233
产品名称
FUT6 Rabbit Polyclonal Antibody (Center)
别名
Alpha-(1,3)-fucosyltransferase 6, Fucosyltransferase 6, Fucosyltransferase VI, Fuc-TVI, FucT-VI, Galactoside 3-L-fucosyltransferase, FUT6, FCT3A
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_000141.1;NP_001035791.1
Human Swissprot No.
P51993
特异性
This FUT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the Central region of human FUT6.
稀释度
WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50
预测分子量
42kDa
同种型
Rabbit Ig
背景介绍
FUT6 is a Golgi stack membrane protein that is involved in the creation of sialyl-Lewis X, an E-selectin ligand.
组织表达
Kidney, liver, colon, small intestine, bladder, uterus and salivary gland
细胞定位
Golgi apparatus, Golgi stack membrane; Single- pass type II membrane protein. Golgi apparatus. Secreted Note=Membrane-bound form in trans cisternae of Golgi
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-FUT6 Antibody (Center) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of FUT6 Antibody (Center) in HL-60 cell line lysates (35ug/lane). FUT6 (arrow) was detected using the purified Pab.

FUT6 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUT6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

FUT6 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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