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ANKFY1 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB2212
产品名称
ANKFY1 Rabbit Polyclonal Antibody (C-term)
别名
Rabankyrin-5, Rank-5, Ankyrin repeat and FYVE domain-containing protein 1, Ankyrin repeats hooked to a zinc finger motif, ANKFY1, ANKHZN, KIAA1255
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_001244928.1;NP_057460.3
Human Swissprot No.
Q9P2R3
特异性
This ANKFY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-465 amino acids from the C-terminal region of human ANKFY1.
稀释度
WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50
预测分子量
128kDa
同种型
Rabbit Ig
背景介绍
ANKFY1 encodes a cytoplasmic protein that contains a coiled-coil structure and a BTB/POZ domain at its N-terminus, ankyrin repeats in the middle portion, and a FYVE-finger motif at its C-terminus. This protein belongs to a subgroup of double zinc finger proteins which may be involved in vesicle or protein transport.
组织表达
High expression in whole adult brain and intermediate expression in all other tissues and specific brain regions examined, including fetal brain.
细胞定位
Cytoplasm. Endosome membrane; Peripheral membrane protein. Early endosome. Note=Also associated with endosomal membranes. Localizes to macropinosomes.
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

ANKFY1 Antibody (C-term) western blot analysis in MDA-MB435,CEM,MCF-7,Jurkat,Hela cell line lysates (35ug/lane).This demonstrates the ANKFY1 antibody detected the ANKFY1 protein (arrow).

ANKFY1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKFY1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ANKFY1 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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