BD-PB2143-Phospho-RAD9(S328) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Phospho-RAD9(S328) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PB2143

产品名称

Phospho-RAD9(S328) Rabbit Polyclonal Antibody

别名

Cell cycle checkpoint control protein RAD9A, hRAD9, DNA repair exonuclease rad9 homolog A, RAD9A

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001230153.1;NP_004575.1

Human Swissprot No.

Q99638

特异性

This RAD9 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S328 of human RAD9.

稀释度

DB~~1:500;WB~~1:1000;IHC-P~~1:100~500

预测分子量

43kDa

同种型

Rabbit Ig

背景介绍

Rad9 is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade.

细胞定位

Nucleus.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphor

Dot blot analysis of anti-Phospho-Rad9-S328 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

The anti-Phospho-Rad9-S328 Pab is used in Western blot to detect Phospho-Rad9-S328 in HL60 (left) and Y79 (right) tissue lysates.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.