BD-PB2140-ATP5B Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

ATP5B Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB2140

产品名称

ATP5B Rabbit Polyclonal Antibody (Center)

别名

ATP synthase subunit beta, mitochondrial, ATP5B, ATPMB, ATPSB

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001677.2

Human Swissprot No.

P06576

特异性

This ATP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 amino acids from the Central region of human ATP5B.

稀释度

IHC-P~~1:100~500;IF~~1:25;WB~~1:1000;FC~~1:10~50

预测分子量

57kDa

同种型

Rabbit Ig

背景介绍

ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.

细胞定位

Mitochondrion inner membrane {ECO:0000250|UniProtKB:P00829}; Peripheral membrane protein {ECO:0000250|UniProtKB:P00829}; Matrix side {ECO:0000250|UniProtKB:P00829}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunohistochemical analysis of paraffin-embedded H. liver section using ATP5B Antibody (Center). BD-PB2140 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. small intestine section using ATP5B Antibody (Center). BD-PB2140 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of SK-BR-3 cells stained with ATP5B Antibody (Center). BD-PB2140 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue) .

Western blot analysis of ATP5B Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATP5B (arrow) was detected using the purified Pab.

Western blot analysis of ATP5B (arrow) using rabbit polyclonal ATP5B Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B gene (Lane 2) .

Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.