BD-PB2135-GSTO1 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

GSTO1 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB2135

产品名称

GSTO1 Rabbit Polyclonal Antibody (Center)

别名

Glutathione S-transferase omega-1, GSTO-1, Glutathione S-transferase omega 1-1, GSTO 1-1, Glutathione-dependent dehydroascorbate reductase, Monomethylarsonic acid reductase, MMA(V) reductase, S-(Phenacyl)glutathione reductase, SPG-R, GSTO1, GSTTLP28

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001177931.1;NP_001177932.1;NP_004823.1

Human Swissprot No.

P78417

特异性

This GSTO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the Central region of human GSTO1.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

28kDa

同种型

Rabbit Ig

背景介绍

GSTO1 is a member of the theta class glutathione S-transferase-like (GSTTL) protein family. In mouse, this protein acts as a small stress response protein, likely involved in cellular redox homeostasis.

组织表达

Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart Lowest expression in brain, placenta and lung

细胞定位

Cytoplasm, cytosol.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Anti-GSTO1 Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of GSTO1 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). GSTO1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with GSTO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

GSTO1 Antibody (Center) flow cytometry analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of GSTO1 (arrow) using rabbit polyclonal GSTO1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTO1 gene.