BD-PB2133-TPI1 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

TPI1 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2133

产品名称

TPI1 Rabbit Polyclonal Antibody (C-term)

别名

Triosephosphate isomerase, TIM, Triose-phosphate isomerase, TPI1, TPI

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_000356.1;NP_001152759.1;NP_001244955.1

Human Swissprot No.

P60174

特异性

This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the C-terminal region of human TPI1.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

27kDa

同种型

Rabbit Ig

背景介绍

TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.

细胞定位

Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU10127}.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Western blot analysis of lysates from A431, HepG2, LNCaP cell line and mouse testis, rat testis tissue lysates (from left to right), using TPI1 Antibody (C-term) . BD-PB2133 was diluted at 1:1000 at each lane.A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

TPI1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.