BD-PB2132-TPI1 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

TPI1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2132

产品名称

TPI1 Rabbit Polyclonal Antibody (N-term)

别名

Triosephosphate isomerase, TIM, Triose-phosphate isomerase, TPI1, TPI

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_000356.1;NP_001152759.1;NP_001244955.1

Human Swissprot No.

P60174

特异性

This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human TPI1.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

27kDa

同种型

Rabbit Ig

背景介绍

TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.

细胞定位

Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU10127}.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

All lanes : Anti-TPI1 Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of TPI1 Antibody (N-term) in Y79(lane 1),CEM(lane 2) cell line and mouse brain tissue(lane 3) lysates (35ug/lane). TPI1 (arrow) was detected using the purified Pab.(2ug/ml)

Western blot analysis of TPI1 (arrow) using rabbit polyclonal TPI1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPI1 gene.

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

TPI1 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.