BD-PB2128-APRT Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

APRT Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2128

产品名称

APRT Rabbit Polyclonal Antibody (C-term)

别名

Adenine phosphoribosyltransferase, APRT, APRT

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_000476.1;NP_001025189.1

Human Swissprot No.

P07741

特异性

This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-170 amino acids from the C-terminal region of human APRT.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

20kDa

同种型

Rabbit Ig

背景介绍

A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.

细胞定位

Cytoplasm.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-APRT Antibody (C-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

APRT Antibody (C-term) western blot analysis in MCF-7 cell line and rat kidney tissue lysates (35ug/lane).This demonstrates the APRT antibody detected the APRT protein (arrow).

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with APRT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.