BD-PB2114-MTHFD1 Rabbit Polyclonal Antibody (Center P550)-现货抗体产品库武汉枢密脑科学技术有限公司

MTHFD1 Rabbit Polyclonal Antibody (Center P550)

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产品基本信息

产品货号

BD-PB2114

产品名称

MTHFD1 Rabbit Polyclonal Antibody (Center P550)

别名

C-1-tetrahydrofolate synthase, cytoplasmic, C1-THF synthase, Methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase, Formyltetrahydrofolate synthetase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, MTHFD1, MTHFC, MTHFD

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_005947.3

Human Swissprot No.

P11586

特异性

This MTHFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-562 amino acids from the Central region of human MTHFD1.

稀释度

WB~~1:1000;IF~~1:10~50;IHC-P~~1:100~500

预测分子量

102kDa

同种型

Rabbit Ig

背景介绍

MTHFD1 is a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.

组织表达

Ubiquitous.

细胞定位

Cytoplasm.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

All lanes : Anti-MTHFD1 Antibody (Center P550) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of MTHFD1 Antibody (Center P550) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of MTHFD1 Antibody (Center P550) in Y79 cell line lysates (35ug/lane). MTHFD1 (arrow) was detected using the purified Pab.

MTHFD1 Antibody (Center P550) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MTHFD1 Antibody (Center P550) for immunohistochemistry. Clinical relevance has not been evaluated.