BD-PB2078-FDPS Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

FDPS Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2078

产品名称

FDPS Rabbit Polyclonal Antibody (N-term)

别名

Farnesyl pyrophosphate synthase, FPP synthase, FPS, (2E,6E)-farnesyl diphosphate synthase, Dimethylallyltranstransferase, Farnesyl diphosphate synthase, Geranyltranstransferase, FDPS, FPS, KIAA1293

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001129293.1;NP_001129294.1;NP_001229753.1;NP_001229754.1;NP_001995.1

Human Swissprot No.

P14324

特异性

This FDPS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-112 amino acids from the N-terminal region of human FDPS.

稀释度

IF~~1:100;IHC-P~~1:100~500;WB~~1:1000

预测分子量

48kDa

同种型

Rabbit Ig

背景介绍

The isoprene biosynthetic pathway supply the cell with cholesterol, ubiquinone, and various nonsterol metabolites. The farnesylpyrophosphate synthetase enzyme catalyzes the formation of geranyl and farnesylpyrophosphate from isopentenylpyrophosphate and dimethylallyl pyrophosphate. Analysis of FDPS activity and protein in rat liver, accompanied by immunofluorescence and immunoelectron microscopy studies, demonstrated that FDPS is predominantly localized in peroxisomes.1 Liver tissue from patients with the peroxisomal deficiency diseases Zellweger syndrome and neonatal adrenoleukodystrophy exhibit diminished activities of FDPS and subsequent isoprenoid synthesis.

细胞定位

Cytoplasm.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunofluorescent analysis of A549 cells, using FDPS Antibody (N-term). BD-PB2078 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of FDPS (arrow) using rabbit polyclonal FDPS Antibody (D31). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FDPS gene.