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MGAT1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号
BD-PB2073
产品名称
MGAT1 Rabbit Polyclonal Antibody (N-term)
别名
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I, GNT-I, GlcNAc-T I, MGAT1, GGNT1, GLCT1, GLYT1, MGAT
类别
常规抗体
推荐应用
WB
反应种属
Human, Mouse
Human Gene ID
NP_001108089.1;NP_001108090.1;NP_001108091.1;NP_001108092.1;NP_002397.2
Human Swissprot No.
P26572
特异性
This MGAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human MGAT1.
稀释度
WB~~1:1000;IHC-P~~1:100~500
预测分子量
51kDa
同种型
Rabbit Ig
背景介绍
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. MGAT1 (UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I) is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis.
细胞定位
Golgi apparatus membrane; Single-pass type II membrane protein. Cytoplasm, perinuclear region. Note=Co-localizes with BRI3 isoform 1 at the perinuclear region.
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

The anti-MGAT1 Pab is used in Western blot to detect MGAT1 in mouse brain tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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