BD-PB2071-AGL Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

AGL Rabbit Polyclonal Antibody (N-term)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB2071

产品名称

AGL Rabbit Polyclonal Antibody (N-term)

别名

Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1,4-1,4-glucantransferase, Amylo-alpha-1,6-glucosidase, Amylo-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, AGL, GDE

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_000019.2;NP_000633.2;NP_000634.2;NP_000635.2;NP_000636.2;NP_000637.2

Human Swissprot No.

P35573

特异性

This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AGL.

稀释度

WB~~1:1000;IF~~1:10~50

预测分子量

175kDa

同种型

Rabbit Ig

背景介绍

AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.

组织表达

Liver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle

细胞定位

Cytoplasm. Note=Under glycogenolytic conditions localizes to the nucleus

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Anti-AGL Antibody (M15) at 1:1000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 175 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Expression of myc-GS causes wild type but not the ÃCBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL ÃCBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.