BD-PB2070-AGL Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

AGL Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2070

产品名称

AGL Rabbit Polyclonal Antibody (C-term)

别名

Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1,4-1,4-glucantransferase, Amylo-alpha-1,6-glucosidase, Amylo-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, AGL, GDE

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_000019.2;NP_000633.2;NP_000634.2;NP_000635.2;NP_000636.2;NP_000637.2

Human Swissprot No.

P35573

特异性

This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1479-1510 amino acids from the C-terminal region of human AGL.

稀释度

WB~~1:1000;IF~~1:10~50

预测分子量

175kDa

同种型

Rabbit Ig

背景介绍

AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.

组织表达

Liver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle

细胞定位

Cytoplasm. Note=Under glycogenolytic conditions localizes to the nucleus

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Anti-AGL Antibody (C-term) at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 175 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot using anti-AGL (C-term) antibody at 1:1000 dilution. A total of 20 ug of lysates was loaded for each tissue. Data courtesy of Dr. Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan.

Expression of myc-GS causes wild type but not the ÃCBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL ÃCBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.

Confocal immunofluorescent analysis of AGL Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).