BD-PB2067-GGA1 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

GGA1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2067

产品名称

GGA1 Rabbit Polyclonal Antibody (N-term)

别名

ADP-ribosylation factor-binding protein GGA1, Gamma-adaptin-related protein 1, Golgi-localized, gamma ear-containing, ARF-binding protein 1, GGA1

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_001001560.1;NP_001001561.1;NP_001166158.1;NP_001166159.1;NP_037497.1

Human Swissprot No.

Q9UJY5

特异性

This GGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GGA1.

稀释度

WB~~1:1000;IF~~1:10~50

预测分子量

70kDa

同种型

Rabbit Ig

背景介绍

GGA1 is a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.

组织表达

Ubiquitously expressed.

细胞定位

Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein Early endosome membrane; Peripheral membrane protein

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

The anti-GGA1 Pab is used in Western blot to detect GGA1 in mouse kidney tissue lysate (lane 1) and Jurkat cell lysate (lane 2).

A, N2a cells cotransfected with APP770 V5 and GGA1 myc or empty vector were immunostained for APP (Alexa488; green), GGA1 (Cy5; blue), and the Golgi marker GM130 (Cy3; red). B, The same transfection was stained for the endosomal marker EEA1 (Cy3). C, To assess cell-surface localization, APP695 GFP and GGA1 myc or empty vector were cotransfected and then immunostained with an Ab to the APP ectodomain on ice without permeabilization (Cy3). Cells were then fixed, permeabilized, and stained for GGA1 (Cy5). (J. Neurosci. 2006 Sep 27;26(39):9913-9922)