BD-PB2056-USP2 Rabbit Polyclonal Antibody (C-term L523)-现货抗体产品库武汉枢密脑科学技术有限公司

USP2 Rabbit Polyclonal Antibody (C-term L523)

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产品基本信息

产品货号

BD-PB2056

产品名称

USP2 Rabbit Polyclonal Antibody (C-term L523)

别名

Ubiquitin carboxyl-terminal hydrolase 2, 41 kDa ubiquitin-specific protease, Deubiquitinating enzyme 2, Ubiquitin thioesterase 2, Ubiquitin-specific-processing protease 2, USP2, UBP41

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_001230688.1;NP_004196.4;NP_741994.1

Human Swissprot No.

O75604

特异性

This USP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 508-538 amino acids from the C-terminal region of human USP2.

稀释度

IHC-P~~1:100~500;WB~~1:2000

预测分子量

68kDa

同种型

Rabbit Ig

背景介绍

USP2, an ubiquitin-specific protease, is selectively up regulated in bone by the osteotropic agents PTH, PTHrP and PGE2 and possibly via the PKA/cAMP pathway. It is also thought to play a role in the recycling of ubiquitin by hydrolysis of branched poly-ubiquitin from linear poly-ubiquitin chains, production of free ubiquitin from linear poly-ubiquitin chains and from certain ribosomal ubiquitin fusion proteins.

组织表达

Expressed in mesangial cells of the kidney and in different types of glomerulonephritides (at protein level)

细胞定位

Cytoplasm {ECO:0000250|UniProtKB:O88623}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:O88623} Note=Localizes in the spermatid head in late-elongating spermatids in the thin area between the outer acrosomal membrane and the plasma membrane. {ECO:0000250|UniProtKB:Q5U349}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

BD-PB2056 staining USP2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/100) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Anti-USP2 Antibody (L314) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human testis tissue reacted with USP2 antibody (C-term L523), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.