BD-PB2050-SMURF1 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

SMURF1 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2050

产品名称

SMURF1 Rabbit Polyclonal Antibody (C-term)

别名

E3 ubiquitin-protein ligase SMURF1, hSMURF1, 632-, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF1, KIAA1625

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_001186776.1;NP_065162.1;NP_851994.1

Human Swissprot No.

Q9HCE7

特异性

This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 711-740 amino acids from the C-terminal region of human SMURF1.

稀释度

WB~~1:500

预测分子量

86kDa

同种型

Rabbit Ig

背景介绍

SMURF1 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and thereby their inactivation.

组织表达

Expressed in melanocytes (PubMed:23999003).

细胞定位

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

All lanes : Anti-SMURF1 Antibody (C-term) at 1:500 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.