BD-PB1939 staining EIF4E2 in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-EIF4E2 Antibody at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: RD whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28, 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.