Overlay histogram showing A431 cells stained with BD-PB1924 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Immunohistochemical analysis of paraffin-embedded R. testis section using Mouse Pbk Antibody(N-term). BD-PB1924 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysate from Hela cell line, using Mouse Pbk Antibody (N-term). BD-PB1924 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot analysis of lysate from mouse testis tissue lysate, using Mouse Pbk Antibody (N-term)at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.