BD-PB1920-Mouse Lats2 Rabbit Polyclonal Antibody(C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

Mouse Lats2 Rabbit Polyclonal Antibody(C-term)

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产品基本信息

产品货号

BD-PB1920

产品名称

Mouse Lats2 Rabbit Polyclonal Antibody(C-term)

别名

Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Lats2 {ECO:0000312|EMBL:AAH530281}

类别

常规抗体

推荐应用

反应种属

Mouse

Human Swissprot No.

Q7TSJ6

Mouse Gene ID

NP_056586.2

特异性

This Mouse Lats2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1000-1027 amino acids from the C-terminal region of mouse Lats2.

稀释度

IHC-P~~1:100;WB~~1:1000

预测分子量

115kDa

同种型

Rabbit Ig

背景介绍

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor (By similarity).

组织表达

Expressed at high levels in ovary and testis and at lower levels in all other tissues examined

细胞定位

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Nucleus. Note=Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis (By similarity). Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment (By similarity)

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunohistochemical analysis of BD-PB1920 on paraffin-embedded Mouse testis tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes : Anti-Mouse Lats2 Antibody(C-term) at 1:2000 dilution Lane 1: Mouse ovary tissue lysate Lane 2: Mouse testis tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 115 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Mouse Lats2 Antibody (C-term)western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Mouse Lats2 antibody detected the Mouse Lats2 protein (arrow).