BD-PB1897-Mouse Trem2 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

Mouse Trem2 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB1897

产品名称

Mouse Trem2 Rabbit Polyclonal Antibody (N-term)

别名

Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2, Trem2, Trem2a, Trem2b, Trem2c

类别

常规抗体

推荐应用

反应种属

Mouse

Human Swissprot No.

Q99NH8

Mouse Gene ID

NP_001259007.1;NP_112544.1

特异性

This Mouse Trem2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-48 amino acids from the N-terminal region of mouse Trem2.

稀释度

IHC-P-Leica~~1:500;WB~~1:500-1000

预测分子量

25kDa

同种型

Rabbit Ig

背景介绍

Trem2 may have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.

组织表达

Expressed in the brain, specifically in microglia (at protein level) (PubMed:15728241, PubMed:28802038, PubMed:28855301, PubMed:28077724, PubMed:28592261, PubMed:29794134, PubMed:29752066, PubMed:27477018, PubMed:28559417). Expressed in macrophages (at protein level) (PubMed:11241283, PubMed:28802038, PubMed:28559417). Expressed at higher levels in the CNS, heart and lung than in lymph nodes or in other non-lymphoid tissues such as kidney, liver and testis (PubMed:12472885). In the CNS not all microglia express TREM2 (PubMed:12472885). Brain regions with an incomplete blood-brain barrier had the lowest percentages of TREM2 expressing microglia, whereas the lateral entorhinal and cingulate cortex had the highest percentages (PubMed:12472885).

细胞定位

[Isoform 1]: Cell membrane; Single-pass type I membrane protein

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunohistochemical analysis of paraffin-embedded mouse lung tissue using BD-PB1897 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using BD-PB1897 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes : Anti-Mouse Trem2 Antibody (N-term) at 1:500-1000 dilution Lane 1: Mouse kidney tissue lysate Lane 2: Mouse testis tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.