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B7H6 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB1836
产品名称
B7H6 Rabbit Polyclonal Antibody (C-term)
别名
Natural cytotoxicity triggering receptor 3 ligand 1, B7 homolog 6, B7-H6, NCR3LG1, B7H6
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_001189368.1
Human Swissprot No.
Q68D85
特异性
This B7H6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-415 amino acids from the C-terminal region of human B7H6.
稀释度
WB~~1:1000
预测分子量
51kDa
同种型
Rabbit Ig
背景介绍
Triggers NCR3-dependent natural killer cell activation.
组织表达
Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B-lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen- transformed cells (at protein level).
细胞定位
Cell membrane; Single-pass type I membrane protein
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-B7H6 Antibody (C-term) at 1:500-1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: Molt-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size : 53kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-B7H6 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: Hela whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-B7H6 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: Hela whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysate from K562 cell line, using B7H6 Antibody (C-term). BD-PB1836 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

B7H6 Antibody (C-term)western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the B7H6 antibody detected the B7H6 protein (arrow).

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