BD-PB1735-DSPP Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

DSPP Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB1735

产品名称

DSPP Rabbit Polyclonal Antibody (N-term)

别名

Dentin sialophosphoprotein, Dentin phosphoprotein, Dentin phosphophoryn, DPP, Dentin sialoprotein, DSP, DSPP

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_055023.2

Human Swissprot No.

Q9NZW4

特异性

This DSPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from the N-terminal region of human DSPP.

稀释度

FC~~1:25;WB~~1:2000

预测分子量

131kDa

同种型

Rabbit Ig

背景介绍

This gene encodes two principal proteins of the dentin extracellular matrix of the tooth. The preproprotein is secreted by odontoblasts and cleaved into dentin sialoprotein and dentin phosphoprotein. Dentin phosphoprotein is thought to be involved in the biomineralization process of dentin. Mutations in this gene have been associated with dentinogenesis imperfecta-1; in some individuals, dentinogenesis imperfecta occurs in combination with an autosomal dominant form of deafness. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq].

组织表达

Expressed in teeth. DPP is synthesized by odontoblast and transiently expressed by pre-ameloblasts

细胞定位

Secreted, extracellular space, extracellular matrix

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Overlay histogram showing PC-3 cells stained with BD-PB1735(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Anti-DSPP Antibody (N-term) at 1:2000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 131 kDa Blocking/Dilution buffer: 5% NFDM/TBST.